GPR158 Antibody: A Key Tool for Neuroscience and Cancer Research

/ My Blog / By

The GPR158 Antibody is a powerful and reliable tool for the detection and study of G protein-coupled receptor 158 (GPR158), a class C orphan GPCR that plays a significant role in the regulation of neuronal signaling and tumorigenesis. GPR158 is implicated in synaptic activity modulation, neuropsychiatric disorders, and cancers, particularly prostate cancer. This antibody offers researchers a high-affinity, versatile tool for understanding the expression, localization, and function of GPR158 in various biological systems.

Key Highlights of GPR158 Antibody

  1. Specific Targeting: Recognizes native and overexpressed levels of GPR158 protein.
  2. Validated Applications: Suitable for Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Enzyme-Linked Immunosorbent Assay (ELISA).
  3. Diverse Reactivity: Reacts with human, mouse, and rat GPR158, making it broadly applicable across multiple model organisms.
  4. High Sensitivity: Polyclonal format ensures detection of various epitopes, offering enhanced signal detection.Biological Significance of GPR158

GPR158 is a class C orphan G-protein coupled receptor that lacks intrinsic enzymatic activity but exerts its biological effects through interactions with intracellular signaling partners.

Roles in Neuroscience

  • Synaptic Function: GPR158 modulates synaptic transmission and plasticity, influencing learning and memory.
  • Mood Disorders: Altered GPR158 levels have been linked to anxiety and depression. GPR158’s regulatory roles in corticosteroid signaling pathways make it a potential therapeutic target.
  • Neurodegeneration: GPR158 may contribute to neurodegenerative processes through dysregulated signaling and inflammation.

Roles in Cancer Biology

  • Prostate Cancer: GPR158 is overexpressed in prostate cancer tissues, where it promotes tumor growth and resistance to apoptosis.
  • Tumor Microenvironment: GPR158 mediates cross-talk between tumor cells and the surrounding stromal cells, influencing angiogenesis and metastasis.

Product Specifications

Attribute Details
Host Species Rabbit
Isotype IgG
Immunogen Synthetic peptide corresponding to GPR158’s C-terminal domain
Applications WB, IHC, IF, ELISA
Reactivity Human, Mouse, Rat
Formulation PBS, pH 7.4, 0.09% sodium azide
Concentration 0.5 mg/mL
Storage Conditions Store at -20°C for long-term use

Applications and Protocols

1. Western Blot (WB)

  • Purpose: Detect GPR158 protein levels in lysates.
  • Protocol:
    1. Run cell or tissue lysates on SDS-PAGE and transfer to a PVDF membrane.
    2. Block with 5% BSA for 1 hour.
    3. Incubate with GPR158 Antibody (1:1,000 dilution) overnight at 4°C.
    4. Wash and incubate with HRP-conjugated secondary antibody.
    5. Visualize using chemiluminescence.

2. Immunohistochemistry (IHC)

  • Purpose: Visualize GPR158 distribution in formalin-fixed, paraffin-embedded tissues.
  • Protocol:
    1. Perform antigen retrieval using citrate buffer (pH 6.0).
    2. Block with 5% serum for 30 minutes.
    3. Incubate with GPR158 Antibody (1:200 dilution) for 1 hour at room temperature.
    4. Apply DAB chromogen for visualization.

3. Immunofluorescence (IF)

  • Purpose: Localize GPR158 in cultured cells or tissue sections.
  • Protocol:
    1. Fix cells with 4% paraformaldehyde and permeabilize with 0.1% Triton X-100.
    2. Block nonspecific binding with 5% BSA.
    3. Incubate with GPR158 Antibody (1:500 dilution) for 1 hour.
    4. Add a fluorescently labeled secondary antibody and counterstain with DAPI.

4. ELISA

  • Purpose: Quantify GPR158 protein in biological samples.
  • Protocol:
    • Use GPR158 Antibody as the capture or detection antibody in a sandwich ELISA format.

Research Areas

  1. Neurobiology: Investigating GPR158’s role in cognitive functions, neuronal health, and neuropsychiatric conditions.
  2. Oncology: Exploring GPR158’s expression patterns and signaling mechanisms in cancer.
  3. Therapeutic Targeting: Evaluating GPR158 as a drug target for mood disorders and cancers.

Advantages of GPR158 Antibody

  1. High Specificity: Minimal cross-reactivity ensures precise detection.
  2. Robust Performance: Polyclonal nature offers high sensitivity in detecting low-abundance proteins.
  3. Broad Compatibility: Validated across multiple applications and model systems.

Storage and Stability

  • Storage: Store at -20°C for long-term use or at 4°C for frequent usage.
  • Stability: Stable for up to 12 months under recommended conditions.

Safety Information

  • Contains sodium azide as a preservative. Handle with appropriate precautions.

References